Journal of the American Chemical Society
Journal of the American Chemical Society
5 years ago

An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins

An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins
Lukas R. Helfinger, Yi Lei Tan, Chih-Ta Henry Chien, Mark J. Bostock, Daniel Nietlispach, Andras Solt
Based on the saposin-A (SapA) scaffold protein, we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins (MPs) under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry, enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of MPs. Three representative MPs, including one G-protein-coupled receptor, were successfully incorporated into SapA-dimyristoylphosphatidylcholine nanoparticles and studied by solution NMR spectroscopy.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b06730

DOI: 10.1021/jacs.7b06730

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