3 years ago

Photoactive Hybrid AuNR-Pt@Ag2S Core–Satellite Nanostructures for Near-Infrared Quantitive Cell Imaging

Photoactive Hybrid AuNR-Pt@Ag2S Core–Satellite Nanostructures for Near-Infrared Quantitive Cell Imaging
Hua Kuang, Liqiang Liu, Liguang Xu, Aihua Qu, Chuanlai Xu, Maozhong Sun
The quantitative detection of microRNA (miR) and multimode-imaging-induced photothermal therapy in vivo have become the focus of much attention. Platinum (Pt) decorated gold nanorods (AuNR-Pt) and Ag2S core–satellite (AuNR-Pt@Ag2S) multifunctional nanostructures are fabricated to quantify intracellular miRs (miR-21), near-infrared fluorescence cell quantitative imaging, and tumor ablation in vivo. When combined with miR-21, the nanoassembly displays significant fluorescence intensity in the second window of the near-infrared region (1000–1700 nm) after 808 nm excitation. The Ag2S fluorescence intensity has a good linear relationship with the amount of intracellular miR in the range of 0.054–20.45 amol ngRNA −1 and a limit of detection of 0.0082 amol ngRNA −1. The nanoassembly is also used to develop multimodal bioimaging, including near-infrared, X-ray computed tomographic, and photoacoustic imaging in HeLa-tumor-bearing mice. Moreover, the tumors are completely eliminated by the high photothermal capacity of the AuNR-Pt@Ag2S assembly. This nanoassembly provides a multifunctional nanoplatform for the ultrasensitive detection of miRs and tumor diagnosis and therapy in vivo. AuNR-Pt@Ag2S multi­functional nanostructures with the yield up to 80% have been fabricated. The Ag2S fluorescent ima­ging in living cells is exhibited to quantify miRNA with a LOD of 0.0082 amol/ngRNA. To be noticed, this is the first reported the Ag2S fluorescent imaging in living cells has been developed.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/adfm.201703408

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