Dynamic landscape and regulation of RNA editing in mammals
To construct a mammalian reference atlas of A-to-I editing, we first compiled a comprehensive list of editing sites in human and mouse (Supplementary Note 1) and then examined the RNA editome across tissues using 8,551 RNA-sequencing (RNA-seq) samples derived from 552 donors in the GTEx project (Supplementary Information 1). Notably, the editing profiles across different tissues were highly correlated (Fig. 1a) and the overall editing activities were also generally similar, except for skeletal muscle, in which editing was significantly lower than in other tissues (P < 2.2 × 10−16, Wilcoxon rank sum test; Fig. 1b). Nevertheless, principal component analysis (PCA) showed that the brain regions could still be resolved from non-brain tissues (Extended Data Fig. 1a). Within the brain, the cerebellum was clearly segregated from other brain parts (Extended Data Fig. 1b), possibly owing to higher expression of ADAR2 (also known as ADARB1) (Extended Data Fig. 1c). When we examined non-repetitive sites in coding regions only, the editing levels became more distinct among the various tissues (Fig. 1a). The different brain regions clustered together, as did heart and skeletal muscle. Unexpectedly, the artery was the most highly edited tissue type (Fig. 1c). The importance of RNA editing in vascular disease was demonstrated in a recent study18. We further validated the results obtained from the GTEx data by applying a targeted sequencing approach (microfluidics-based multiplex PCR and deep sequencing; mmPCR–seq)19 (Supplementary Note 2) to examine 1
Publisher URL: http://dx.doi.org/10.1038/nature24041
Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.
Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.