3 years ago

Optimizing Human Induced Pluripotent Stem Cell Expansion in Stirred Suspension Culture.

Liu, Poon, Meng, Rancourt
Human induced pluripotent stem cells (hiPSCs) hold great hopes for application in regenerative medicine due to their inherent capacity to self-renew and differentiate into cells from the three embryonic germ layers. For clinical applications, a large quantity of hiPSCs produced in standardized and scalable culture processes is required. Several groups including ours have developed methodologies for scaled-up hiPSC production in stirred bioreactors in chemically defined medium. Here, we optimized the critical steps and factors that affect hiPSC expansion and yield in stirred suspension cultures including inoculation conditions, seeding density, aggregate size, agitation rate, and cell passaging method. After multiple passages in stirred suspension bioreactors, hiPSCs remained pluripotent, karyotypically normal, and capable of differentiating into all three germ layers.

Publisher URL: http://doi.org/10.1089/scd.2017.0090

DOI: 10.1089/scd.2017.0090

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