Frontiers in Microbiology
Frontiers in Microbiology
3 years ago

Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings.

Nitsche, Kohl, Kurth, Wegener
Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater(®) -treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater(®) buffer yield similar results to those stored snap-frozen.

Publisher URL: http://doi.org/10.3389/fmicb.2017.01888

DOI: 10.3389/fmicb.2017.01888

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.