Molecular Cell
Molecular Cell
5 years ago

Regulation of m6A Transcripts by the 3ʹ→5ʹ RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline

Regulation of m6A Transcripts by the 3ʹ→5ʹ RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline
N 6 -methyladenosine (m6A) is an essential internal RNA modification that is critical for gene expression control in most organisms. Proteins with a YTH domain recognize m6A marks and are mediators of molecular functions like RNA splicing, mRNA decay, and translation control. Here we demonstrate that YTH domain-containing 2 (YTHDC2) is an m6A reader that is essential for male and female fertility in mice. High-throughput mapping of the m6A transcriptome and expression analysis in the Yhtdc2 mutant testes reveal an upregulation of m6A-enriched transcripts. Our biochemical studies indicate that YTHDC2 is an RNA-induced ATPase with a 3ʹ→5ʹ RNA helicase activity. Furthermore, YTHDC2 recruits the 5ʹ→3ʹ exoribonuclease XRN1 via Ankyrin repeats that are inserted in between the RecA modules of the RNA helicase domain. Our studies reveal a role for YTHDC2 in modulating the levels of m6A-modified germline transcripts to maintain a gene expression program that is conducive for progression through meiosis.

Graphical abstract

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Teaser

Wojtas et al. show that the cytoplasmic m6A “reader” and 3ʹ→5ʹ RNA helicase YTHDC2 is essential for regulating m6A transcripts to ensure a successful meiotic gene expression program in the mammalian germline, and that this is essential for male and female fertility.

Publisher URL: www.sciencedirect.com/science

DOI: S1097276517306998

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