3 years ago

Biosynthesis of Chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach

Biosynthesis of Chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach
Currently applied methodologies (acidic or enzymatic chitosanolysis) towards synthesis of Chitosan- Oligosaccharides (COS) are faced with some drawbacks that confine their potential in biotechnological applications. The present work addresses a novel tailored method as an alternate to biosynthesize COS. An empirical statistical-mathematical approach [Plackett-Burman. Design (PBD) followed by Box-Behnken design (BBD)] was employed to optimize COS biosynthesis by an endo-chitosanase non-aflatoxigenic producing Aspergillus sp. section Flavi strain EGY1 DSM 101520 whole cells. Optimal levels localized by BBD for three key determinants were 0.687% (w/v) low molecular weight chitosan (LMWC), 25°C incubation temperature and 72–96h incubation time, along with 2.0g.L−1 maximal level of COS, achieving 94.7% model adequacy upon growing the fungus on LMWC-based tap water medium [merely 0.687% (w/v) LMWC]. Batch mode scaling up in the laboratory scale fermenter achieved 5.34g.L−1 of COS after 44h with 2.67 fold enhancement. Partially acetylated hetero-oligomers with DPs (2-11) of the water-soluble COS were proved by TLC, MALDI-TOF-MS, 1H NMR, 13C NMR and FTIR. To the best of the authors' knowledge, the present work is the first study highlighting a novel, robust, cheap, environmentally safe, reproducible approach to biosynthesize COS by fungal whole cells.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511316310674

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.