3 years ago

Implementation of buffy-coat-derived pooled platelet concentrates for internal quality control of light transmission aggregometry: a proof of concept study

D. von Lewinski, EC. Weiss, H. Mangge, K. Rosskopf, F. Prüller, D. Enko, A. Riegler, E. Mahla
Background Standardized commercially available control material for internal quality control (IQC) of light transmission aggregometry (LTA) is still lacking. Moreover, the provision of normal blood donors to provide fresh platelets is difficult in small laboratories, where “volunteers” may be of short supply. Objectives To evaluate the implementation of buffy-coat-derived pooled platelet concentrates (PCs) for IQC material of LTA. Methods We used buffy-coat-derived pooled PCs from the blood bank as IQC material for LTA. On each weekend one PC was prepared (> 200 mL) and aliquoted from the original storage bag on a daily basis in four baby bags (40 – 50 mL), which were delivered from Monday to Friday to our laboratory. The IQC measurements of at least 85 workweeks (from Monday to Friday) were evaluated with this new IQC material. LTA was performed on a four channel Chronolog 700 Aggregometer (Chronolog Corporation, Havertown, Pennsylvania, USA) (agonists: collagen, adenosine diphosphate [ADP], arachidonic acid [AA], thrombin receptor activator peptide-6 [TRAP-6]). Results The medians of platelet aggregation from IQC measurements were with collagen, ADP, and AA from Monday to Friday 68.0 – 59.5, 3.0 – 2.0, and 51.0 – 50.0%, and the mean of platelet aggregation with TRAP-6 71.2 – 66.4%, respectively. Conclusions Buffy-coat-derived pooled PCs serve as a reliable and robust IQC material for LTA measurements and would be beneficial for the whole laboratory procedure and employees’ safety. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/jth.13870

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