Biotechnology Journal
Biotechnology Journal
5 years ago

Label-free multiplex DNA detection utilizing projected capacitive touchscreen

Byoung Yeon Won, Joon Young Lee, Hyun Gyu Park
We have developed a novel strategy to achieve label-free multiplex DNA detection system based on the projected capacitive touchscreen. Touchscreen panel was first fabricated by patterning the ITO (indium-tin-oxide) electrode array on the glass wafer, and the electrodes were modified with the respective capture probe DNA sequences complementary to hemagglutinin1 (H1), neuraminidase1 (N1), and matrix1 (M1) DNA to demonstrate the molecular diagnosis of H1N1 influenza virus as a model pathogen. DNA sample was then applied to the electrodes to allow hybridization of target DNA with the corresponding complementary capture probe. As a result, the hybridization event significantly enhanced the capacitance on the electrode, which can be very conveniently detected by the projected capacitive touchscreen. Based on this design principle, we have successfully detected target regions of H1N1 influenza virus in a label-free multiplexed manner. This touchscreen-based detecting system would greatly benefit the point-of-care testing (POCT) in various diagnostic applications.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/biot.201700362

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