Pest Management Science
Pest Management Science
4 years ago

Characterization of UDP-glucuronosyltransferase genes and their possible roles in multi-insecticide resistance in Plutella xylostella (L.)

Xiuxia Li, Xiwu Gao, Pei Liang, Haiyan Shi
BACKGROUND Uridine diphosphate-glucuronosyltransferases (UGTs), as multifunctional detoxification enzymes, play important roles in the biotransformation of various compounds. However, their roles in insecticide resistance are still unclear. This study presents a genome-wide identification of the UGTs in diamondback moth, Plutella xylostella (L.), a worldwide notorious insect pest of cruciferous crops. The possible roles of these UGTs in insecticide resistance were evaluated. RESULTS A total of 21 putative UGTs in P. xylostella were identified. Quantitative real-time PCR-based analyses showed that all the UGT genes were expressed in all tested developmental stages and tissues. Bioassay results indicated that a field-collected population (BL) was resistant to 9 of 10 commonly used insecticides, and 10 of 21 UGT mRNAs were up-regulated in the BL population. Exposure to the LC50 of each insecticide affected the expression of most UGT genes. Among these, the expression levels of UGT40V1, UGT45B1 and UGT33AA4 were induced by more than five insecticides, while indoxacarb and metaflumizone significantly repressed the expression of most UGT genes. CONCLUSION UGTs may play important roles in the metabolism of commonly used insecticides in P. xylostella. These findings provide valuable information for further research on the physiological and toxicological functions of specific UGT genes in P. xylostella.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/ps.4765

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