4 years ago

Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor

Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor
Steven M. Pauff, William R. Kobertz, Stephen C. Miller, H. M. Dhammika Bandara, Elizabeth A. Colby Davie, Zhengmao Hua, Mei Zhang
Potassium (K+) exits electrically excitable cells during normal and pathophysiological activity. Currently, K+-sensitive electrodes and electrical measurements are the primary tools to detect K+ fluxes. Here, we describe the synthesis of a near-IR, oxazine fluorescent K+ sensor (KNIR-1) with a dissociation constant suited for detecting changes in intracellular and extracellular K+ concentrations. KNIR-1 treatment of cells expressing voltage-gated K+ channels enabled the visualization of intracellular K+ depletion upon channel opening and restoration of cytoplasmic K+ after channel closing.

Publisher URL: http://dx.doi.org/10.1021/acs.joc.7b00845

DOI: 10.1021/acs.joc.7b00845

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