3 years ago

Efficient 31P band inversion transfer approach for measuring creatine kinase activity, ATP synthesis, and molecular dynamics in the human brain at 7 T

A. Dean Sherry, Craig R. Malloy, Jimin Ren
Purpose To develop an efficient 31P magnetic resonance spectroscopy (MRS) method for measuring creatine kinase (CK) activity, adenosine triphosphate (ATP) synthesis, and motion dynamics in the human brain at 7 Tesla (T). Methods Three band inversion modules differing in center frequency were used to induce magnetization transfer (MT) effect in three exchange pathways: (i) CK-mediated reaction PCr γ-ATP; (ii) de novo ATP synthesis Pi γ-ATP; and (iii) ATP intramolecular 31P–31P cross-relaxation γ-(α-) β-ATP. The resultant MT data were analyzed using a 5-pool model in the format of magnetization matrix according to Bloch-McConnell-Solomon formalism. Results With a repetition time (TR) of 4 s, the scan time for each module was approximately 8 min. The rate constants were kPCr γATP 0.38 ± 0.02 s−1, kPi γATP 0.19 ± 0.02 s−1, and σγ(α) βATP 0.19 ± 0.04 s−1, corresponding to ATP rotation correlation time τc (0.8 ± 0.2) ·10−7 s. The T1 relaxation times were Pi 7.26 ± 1.76 s, PCr 5.99 ± 0.58 s, γ-ATP 0.98 ± 0.07 s, α-ATP 0.95 ± 0.04 s, and β-ATP 0.68 ± 0.03 s. Conclusion Short-TR band inversion modules provide a time-efficient way of measuring brain ATP metabolism and could be useful in studying metabolic disorders in brain diseases. Magn Reson Med 78:1657–1666, 2017. © 2016 International Society for Magnetic Resonance in Medicine.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mrm.26560

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