3 years ago

Retrospective proteomic analysis of serum after Akhmeta virus infection: new suspect case identification and insights into poxvirus humoral immunity.

Gallardo-Romero, Townsend, Satheshkumar, Khmaladze, Reynolds, Geleishvili, Emerson, Carroll, Vora, Maghlakelidze
Serologic cross-reactivity, a hallmark of orthopoxvirus (OPXV) infection, makes species-specific diagnosis of infection difficult. In this study, we used a Variola virus (VARV) proteome microarray to characterize and differentiate antibody responses to non-vaccinia OPXV infections from smallpox vaccination. The profile of two-case patients infected with newly discovered OPXV, Akhmeta virus (AKMV), exhibited antibody responses of greater intensity and broader recognition of viral proteins and includes the B21/22 family glycoproteins not encoded by vaccinia virus (VACV) strains used as vaccines. An additional case of AKMV, or non-vaccinia OPXV infection, was identified from community surveillance of individuals with no or uncertain history of vaccination and no recent infection. The results demonstrate the utility of microarrays for high resolution mapping of antibody response to determine nature of OPXV exposure.

Publisher URL: http://doi.org/10.1093/infdis/jix534

DOI: 10.1093/infdis/jix534

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