Endocrinology
Endocrinology
5 years ago

Serum quantitative proteomic analysis reveals soluble EGFR to be a marker of insulin resistance in male mice and humans.

Okuyama, Kyohara, Terauchi, Togashi, Shirakawa, Hirano, Tajima, Kimura
To identify circulating factors as candidates involved in type 2 diabetes (T2DM), we conducted two different quantitative proteomic analyses: 1) db/db mouse sera were compared with db/+ mouse sera obtained at 4, 8, 12, and 24 weeks of age, and 2) db/db mouse sera from animals treated with liraglutide were compared with sera from animals without liraglutide treatment. 20 and eight proteins were differentially expressed in db/db mouse sera in the first experiment and in db/db mouse sera after liraglutide treatment in the second experiment, respectively. Soluble epidermal growth factor receptor (sEGFR) was identified as a common factor, and its protein level was significantly affected in both experiments. An ELISA assay confirmed that the relatively low serum sEGFR levels in db/db mice were restored by liraglutide treatment. The serum sEGFR levels were elevated in diabetic mice with impaired insulin secretion and decreased in high-fat diet-fed mice and ob/ob mice. The serum sEGFR levels increased after the administration of a dual inhibitor of IGF-1/insulin receptor or streptozotocin. In humans with normal glucose tolerance or T2DM, the serum sEGFR levels were correlated with the fasting blood glucose, fasting serum insulin, HOMA-IR, HbA1c, total cholesterol, LDL-cholesterol, and triglycerides levels. These findings suggest that sEGFR might be a biomarker for evaluating insulin resistance or a therapeutic target of liraglutide.

Publisher URL: http://doi.org/10.1210/en.2017-00339

DOI: 10.1210/en.2017-00339

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.