5 years ago

Development of bufferless gel electrophoresis chip for easy preparation and rapid DNA separation

Nam Ho Bae, Seok Jae Lee, Younghee Kim, Sungsu Park, Sergiy Oleksandrov, Kyoung G. Lee, Wanyoung Lim, Abdurazak Aman
This work presents a handy, fast, and compact bufferless gel electrophoresis chip (BGEC), which consists of precast agarose gel confined in a disposable plastic body with electrodes. It does not require large volumes of buffer to fill reservoirs, or the process of immersing the gel in the buffer. It withstands voltages up to 28.4 V/cm, thereby allowing DNA separation within 10 minutes with a similar separation capability to the standard gel electrophoresis. The results suggest that our BGEC is highly suitable for in situ gel electrophoresis in forensic, epidemiological settings and crime scenes where standard gel electrophoresis equipment cannot be brought in while quick results are needed. This article is protected by copyright. All rights reserved

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/elps.201700326

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