Mikrochimica Acta
Mikrochimica Acta
3 years ago

Visual and rapid lateral flow immunochromatographic assay for enrofloxacin using dyed polymer microspheres and quantum dots

Yue Liu, Wei Sheng, Shuo Wang, Junping Wang, Yan Zhang, Shijie Li

Abstract

The authors describe two kinds of immunochromatographic assays. The first is based on the use of dyed polymer microspheres (MICA), the another on the use of quantum dots (QICA). Both enable visual detection of enrofloxacin (ENR) in animal tissue and milk. Both the MICA and the QICA have visual limits of detection of 1 μg·L−1 when working in buffer, of 5 μg·kg−1 in case of animal tissue, and of 10 μg·L−1 in case of milk. Other quinolones do not interfere. The MICA and QICA described here are convenient and fairly rapid in that the detection process (including sample pretreatment and assay) takes 20 min only which is far less than the commercial enzyme-linked immunosorbent assay (ELISA) test kit which requires 120 min. The MICA and QICA are more sensitive than the immunochromatographic assay using colloidal gold labels and the same polyclonal antibody. The results of analysis of spiked samples via MICA and QICA are in good agreement with those obtained by the commercial ELISA test kit.

Graphical abstract

A visual dyed polymer microsphere-based immunochromatographic assay (MICA) and a visual quantum-dot-based immunochromatographic assay (QICA) are described for the rapid, specific, qualitative, semi-quantitative, and on-site detection of enrofloxacin residues in animal origin foods.

Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2474-y

DOI: 10.1007/s00604-017-2474-y

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