3 years ago

A glassy carbon electrode modified with graphene nanoplatelets, gold nanoparticles and chitosan, and coated with a molecularly imprinted polymer for highly sensitive determination of prostate specific antigen

Li-Shi Wang, Qiang Zeng, Ya Ma, Xiao-Lei Shen


The authors describe an amperometric assay for the detection of prostate specific antigen (PSA) that combines the advantages of using a molecularly imprinted polymer (MIP) and of a nanocomposite composed of  graphene nanoplatelets (graphene sheets; GS), gold nanoparticles (AuNPs) and chitosan (Chit). The GS-AuNP composite was synthesized by a single-step reduction of GS and HAuCl4 solution. The MIP was synthesized by electropolymerization of dopamine and characterized by scanning electron microscopy and differential pulse voltammetry (DPV). Sensitivity is strongly improved by the magnified current obtained by using the GS-AuNP hybrid. Chit was further employed as a film-forming material to prevent the leakage of nanomaterials. Under optimized conditions, the method displays good analytical performance for the detection of PSA by DPV and by using hexacyanoferrate as the electrochemical probe. The peak current (typically measured at 0.16 V vs. SCE) increases linearly in the 1 pg mL−1 to 100 ng mL−1 PSA concentration range, and the detection limit is 0.15 pg mL−1 at a signal to noise ratio of 3. The method was successfully applied to the determination of PSA in serum. The assay is highly selective, sensitive, reproducible and stable. In our perception, it represents an attractive alternative to the commercially available ELISA kits for PSA.

Graphical abstract

Schematic of a molecularly imprinted material obtained via electropolymerization of dopamine onto gold nanoparticles and placed, along with graphene nanoplatelets and chitosan on an electrode to detect prostate specific antigen (PSA). The sensor shows good specificity and accuracy for PSA detection in female serum.

Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2458-y

DOI: 10.1007/s00604-017-2458-y

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.