An antibacterial microfluidic system with fish gill structure for the detection of Staphylococcus via enzymatic reaction on a chromatic polydiacetylene material caused by lysostaphin
The authors describe a microfluidic system functionalized with a chromatic nanomaterial (polydiacetylene; PDA) and conjugated to the antimicrobial enzyme lysostaphin (LST) as a means for specific detection of Staphylococcus pathogens and to simultaneously perform antimicrobial functions. The LST-loaded PDA vesicles were deposited in a fish gill-like structure on the inner surface of the microchannels. They undergo a color transition from blue to red and enhancement of fluorescence under external mechanical stimulus that is caused by the interaction between Staphylococcus and LST which has antimicrobial activity against Staphylococcus. Due to its fish gill-mimicking structure, the PDA coated channel has a high surface-to-volume ratio, and this maximizes the binding efficiency between Staphylococcus suspended in the fluid and the LST-PDA coating on the microchannels. Consequently, >80% of the Staphylococcus are eliminated in the channels within a short reaction time. As a result, the LST-PDA-coated channel surfaces undergoes color change from blue to red, and red fluorescence pops up. In contrast, no enzymatic reaction and no color transition is observed when an E. coli suspension is applied. The results show that this multifunctional microfluidic system can specifically detect, and can exert an antimicrobial effect on, Staphylococcus.
Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2517-4
Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.
Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.