An antibacterial microfluidic system with fish gill structure for the detection of Staphylococcus via enzymatic reaction on a chromatic polydiacetylene material caused by lysostaphin
Abstract
The authors describe a microfluidic system functionalized with a chromatic nanomaterial (polydiacetylene; PDA) and conjugated to the antimicrobial enzyme lysostaphin (LST) as a means for specific detection of Staphylococcus pathogens and to simultaneously perform antimicrobial functions. The LST-loaded PDA vesicles were deposited in a fish gill-like structure on the inner surface of the microchannels. They undergo a color transition from blue to red and enhancement of fluorescence under external mechanical stimulus that is caused by the interaction between Staphylococcus and LST which has antimicrobial activity against Staphylococcus. Due to its fish gill-mimicking structure, the PDA coated channel has a high surface-to-volume ratio, and this maximizes the binding efficiency between Staphylococcus suspended in the fluid and the LST-PDA coating on the microchannels. Consequently, >80% of the Staphylococcus are eliminated in the channels within a short reaction time. As a result, the LST-PDA-coated channel surfaces undergoes color change from blue to red, and red fluorescence pops up. In contrast, no enzymatic reaction and no color transition is observed when an E. coli suspension is applied. The results show that this multifunctional microfluidic system can specifically detect, and can exert an antimicrobial effect on, Staphylococcus.
Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2517-4
DOI: 10.1007/s00604-017-2517-4
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