3 years ago

A portable microreactor with minimal accessories for polymerase chain reaction: application to the determination of foodborne pathogens

Nae Yoon Lee, Kieu The Loan Trinh

Abstract

The authors describe a microreactor for performing a multiplexed polymerase chain reaction (PCR) which is operated with minimal accessories such as a single heater for gene amplification and a hand-held syringe for sample actuation. It was fabricated by wrapping a polytetrafluoroethylene (PTFE) tube around a rectangular poly(dimethylsiloxane) (PDMS) block having a predetermined thickness. The resulting portable microreactor was applied to PCR using a single heater because the rectangular PDMS block spatially segregated the upper and lower arrays of the PTFE tube. This warrants the adjustment of distinct temperatures inside the upper and lower tubes. A hand-held plastic syringe was connected to the inlet of the PTFE tube and used as a portable pump to achieve a homogeneous transport of a sample liquid inside the microreactor. The use of gas-impermeable PTFE prevents the formation of bubbles during thermal cycling. The microreactor was successfully applied to the amplification of typical DNA fragments of three foodborne pathogens in less than 30 min. In our perception, this method paves the way to the construction of a truly portable PCR chip that is applicable to rapid clinical diagnosis and the detection of foodborne pathogens.

Graphical abstract

Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2451-5

DOI: 10.1007/s00604-017-2451-5

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.