3 years ago

A nanocomposite containing Prussian Blue, platinum nanoparticles and polyaniline for multi-amplification of the signal of voltammetric immunosensors: highly sensitive detection of carcinoma antigen 125

Zhanfang Ma, Yun Zheng, Huiqiang Wang


The authors describe a method for signal amplification of label-free voltammetric immunosensors. A glassy carbon electrode (GCE) was modified with Prussian Blue-platinum nanoparticles (PB-PtNPs) as a redox-active species that gives a strong amperometric signal at 0.18 V (vs. Ag/AgCl). Benefitting from the excellent electrical conductivity and the strong catalytic activity to H2O2, the modified GCE gives a strongly enhanced signal. The PB-PtNPs were incorporated into a polyaniline (PANI) hydrogel to further enhance the signal. The signal response of the PB-PtNP-PANI/GCE is larger by a factor of 7.6 than that of PB-PtNP/GCE. In order to further improve electrical conductivity and immobilize antibody, gold nanoparticles (AuNPs) were deposited on the surface of the PB-PtNP-PANI hydrogel. The AuNP-PB-PtNP-PANI hydrogel nanocomposite on the GCE was used in an immunosensor for the model analyte carcinoma antigen 125 (CA125), a biomarker for epithelial ovarian cancer, by immobilizing the respective antibody on the modified GCE. A linear response found for the 0.01 to 5000 U mL−1 CA125 concentration range, with a detection limit of 4.4 mU mL−1 (at an S/N ratio of 3). The electrochemical sensitivity is as high as 119.76 μA·(U/mL)−1·cm−2. The detection of CA125 in human serum showed satisfactory accuracy compared to a commercial chemiluminescent microparticle immunoassay (CMIA).

Graphical abstract

Schematic of a nanocomposites consisting of gold nanoparticles, Prussian Blue, platinum nanoparticles and polyaniline hydrogel as a signal multi-amplification sensing substrate for the ultrasensitive immuno detection of carcinoma antigen 125 (CA125).

Publisher URL: https://link.springer.com/article/10.1007/s00604-017-2470-2

DOI: 10.1007/s00604-017-2470-2

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.