3 years ago

Development of a Sensor Based on Modified Carbon Paste with Com Iron(III) Protoporphyrin Immobilized on SiNbZn Silica Matrix for L-tryptophan Determination

Development of a Sensor Based on Modified Carbon Paste with Com Iron(III) Protoporphyrin Immobilized on SiNbZn Silica Matrix for L-tryptophan Determination
Arnaldo César Pereira, César Ricardo Teixeira Tarley, Daniela Nunes da Silva
In this work, SiO2/Nb2O5/ZnO prepared by the sol-gel processing method was used as substrate base for immobilization of the protoporphyrin-IX ion. Iron(III) ion was inserted into the porphyrin ring (SiNbZn-PPFe). A simple square wave voltammetry method based on a composite sensor carbon paste electrode of this material,designed as EPC-SiNbZn-PPFe, was developed and validated successfully for the determination of L-tryptophan (Trp). The optimum conditions were obtained by using sensor modified with 18.00 mg SiNbZn-PPFe material, 12.00 mg graphite powder and 6.0 μL mineral oil and phosphate buffer 0.3 mol L−1 pH 7.0. The sensitivity of the sensor was found to be 0.523 AL mol −1, linear range from 10 to 70 μmol L−1 and limit of detection of 3.28 μmol L−1. Therefore, the developed method was successfully applied for the Trp determination in real samples of pharmaceutical formulation and can be used for routine quality control pharmaceutical formulations containing Trp.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/elan.201700476

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