Electroanalysis
Electroanalysis
5 years ago

Amperometric L-cysteine Sensor Using a Gold Electrode Modified with Thiolated Catechol

Amperometric L-cysteine Sensor Using a Gold Electrode Modified with Thiolated Catechol
Dongcheng Song, Chaorong Li, Zhaohong Su, Yan Zhao, Xiaolin Xu, Xiaoying Liu, Haitao Xu
A thiolated catechol (CA) consisting of 1,6-Hexanedithiol (HDT) and CA was modified on a gold (Au) electrode to obtain an amperometric L-cysteine sensor with detection limit of 60.6 nM. The preparation of thiolated CA was conducted via a thiol addition between HDT and electro-oxidized CA (EOCA). Briefly, the thiol addition reaction was accomplished by potential cycling of HDT/Au electrode in 0.1 M phosphate buffer (PB, pH 7.2) containing CA, and an EOCA-HDT/Au electrode was produced. The obtained EOCA-HDT/Au electrode exhibits a pair of well-defined redox peaks (at 0.22/0.10 V) of o-quinone moiety, which effectively mediates the oxidation of L-cysteine in a 0.1 M PB (pH 7.2), with an over-potential decrease by ca. 0.12 V (versus bare Au electrode). Electrochemical quartz crystal microbalance, cyclic voltammetry and surface-enhanced Raman spectra were used to study relevant processes and/or film properties. The amperometric L-cysteine sensor has good anti-interferent ability and reproducibility. It also has acceptable recovery for detection of L-cysteine in urine samples.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/elan.201700162

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