3 years ago

Simultaneous electrochemical determination of isoniazid and ethambutol using poly-melamine/electrodeposited gold nanoparticles modified pre-anodized glassy carbon electrode

Elias Ranjbari, Zahra Sepehri, Mohaddeseh Amiri-Aref, Hasan Bagheri, Yalda Hosseinzadeh Ardakani, Mohammad Reza Rouini, Salimeh Amidi

Abstract

For the first time, simultaneous voltammetric determination of two kinds of the first-line categorized anti-tuberculosis drugs including isoniazid (INZ) and ethambutol (EBL) was reported at a highly sensitive electrochemical sensor. The proposed sensor was successfully prepared based on an electroactive poly-melamine film and electrodeposited gold nanoparticles (PMel-Aunano)-modified pre-anodized glassy carbon electrode (GCE*). The morphological and electrochemical characteristics of the sensing surface (PMel-Aunano/GCE*) was well-characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The PMel-Aunano/GCE* exhibited strongly catalytic activity toward the oxidation of INZ (0.39 V potential shift) and EBL (0.29 V potential shift) in comparison with PMel/GCE* and bare GCE. Differential pulse voltammograms of INZ and EBL depicted the linear responses with their concentrations at the ranges of 0.3 to 170.0 μM and 0.5 to 150.0 μM, respectively. The detection limits for INZ and EBL were sequentially estimated as 0.08 and 0.21 μM. Furthermore, the developed electrochemical sensor was successfully implemented for the determination of INZ and EBL in real samples using standard addition method. This fabricated sensor showed to be promising for INZ and EBL detection with many desirable features including high-sensitivity, low detection limit, decrease in over-voltage, reproducible responses, and acceptable anti-interferences ability.

Publisher URL: https://link.springer.com/article/10.1007/s11581-017-2263-y

DOI: 10.1007/s11581-017-2263-y

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.