3 years ago

LAPS and SPIM Imaging Using ITO-Coated Glass as the Substrate Material

LAPS and SPIM Imaging Using ITO-Coated Glass as the Substrate Material
Steffi Krause, De-Wen Zhang, Fan Wu
Light-addressable potentiometric sensors (LAPS) and scanning photo-induced impedance microscopy (SPIM) use photocurrent measurements for spatiotemporal imaging of ion concentrations, electrical potentials, and impedance. In this work, ITO-coated glass was confirmed to produce photocurrents at anodic potentials with 405 nm diode laser illumination. Therefore, it was developed as a low cost and robust substrate material for LAPS and SPIM imaging compared to traditional expensive ultrathin Si substrates. ITO showed good ac photocurrent and pH response without surface modification and insulator. Local photocurrents were produced by scanning a focused laser beam across the sample, which proved the light addressability of ITO-coated glass. With a high-impedance PMMA dot deposited onto the ITO as a model system, a lateral resolution of about 2.3 μm was achieved.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b01898

DOI: 10.1021/acs.analchem.7b01898

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