Chemistry - A European Journal
Chemistry - A European Journal
5 years ago

PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis

PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis
Ciara K. O'Sullivan, Bernold Hasenknopf, Richard B. Cole, Serge Thorimbert, Ahmed M. Debela, Mayreli Ortiz, Denis Lesage, Marketa Svobodova
Redox-labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase-mediated incorporation while also being electrochemically discriminable. The synthesis and characterization of Keggin and Dawson polyoxometalate–deoxynucleotide (POM–dNTP) bioconjugates linked through 7-deaza-modified purines is described. The modified POM–dNTPs were used for polymerase-based amplification of a DNA sequence specific for Yersinia pestis and the amplified DNA detected using an electrochemical DNA sensor. This highlights the potential of polyoxometalates as thermally stable, sensitive and polymerase-compatible redox labels for exploitation in bioanalytical applications. Decorating DNA: Keggin and Dawson polyoxometalate–deoxynucleotide (POM–dNTP) bioconjugates were used for decorating a DNA sequence through a polymerase-based amplification. This amplified DNA product was detected using differential pulse voltammetry.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201701295

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