3 years ago

Discovery of a Turn-On Fluorescent Probe for Myeloid Cell Leukemia-1 Protein

Discovery of a Turn-On Fluorescent Probe for Myeloid Cell Leukemia-1 Protein
Minyong Li, Yichao Wan, Tingting Liu, Hao Fang, Lupei Du, Xiaomeng Zhang, Yuqi Gao
The environment-sensitive probe 2 with turn-on switch for Mcl-1 protein was developed herein. After careful evaluation, this small molecule fluorescent probe revealed a selective binding affinity with Mcl-1 protein with a Ki value of 2.6 μM and can be well applied to imaging Mcl-1 protein or detecting the cellular distribution of Mcl-1 protein inhibitors. Compared with other imaging approaches, such as the immunofluorescence and fluorescent protein-based techniques, this fluorescent method is rapid, convenient, and affordable, especially since a washing procedure is no longer needed. This environment-sensitive “off-on” design strategy may present a case in point for developing small-molecule fluorescent probe of Bcl-2 family proteins.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b01148

DOI: 10.1021/acs.analchem.7b01148

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