Applied and Environmental Microbiology
Applied and Environmental Microbiology
5 years ago

Common presence of aerobic anoxygenic photosynthesis within the genus Limnohabitans.

Michal Koblížek, Yonghui Zeng, Karel Šimek, Vojtěch Kasalický, Hana Kratochvilová, Kasia Piwosz
The genus Limnohabitans (Comamonadaceae, Betaproteobacteria) is a common and a highly active component of freshwater bacterioplankton communities. So far, the genus has been considered to contain only heterotrophic species. In this study, we detected photosynthesis genes pufLM and bchY in 28 out of 46 strains from three Limnohabitans lineages. The obtained pufM sequences are very closely related to environmental pufM sequences detected in various freshwater habitats, indicating ubiquity and potential importance of photoheterotrophic Limnohabitans in nature.Additionally, we sequenced and analyzed the genomes of 5 potentially photoheterotrophic Limnohabitans strains to gain further insights into their phototrophic capacity. The structure of the photosynthesis gene cluster turned out to be highly conserved within the genus Limnohabitans and also among all potentially photosynthetic Betaproteobacteria The expression of photosynthetic complexes was detected in a culture of Limnohabitans planktonicus II-D5(T) using spectroscopic and pigment analyses. This was further verified by a novel combination of infra-red microscopy with fluorescent in situ hybridization.Importance The presented data document that the capacity to perform anoxygenic photosynthesis is common among the members of genus Limnohabitans, indicating that they may have a novel role in freshwater habitats.

Publisher URL: http://doi.org/10.1128/AEM.02116-17

DOI: 10.1128/AEM.02116-17

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