5 years ago

Using SDS-PAGE gel finger printing to identify soft bodied wood boring insect larvae to species

Daniel Reed, Mark A O'Neill, Mia Denos
BACKGROUND This paper describes the progress which we have made assessing the feasibility of “finger printing” using imaged SDS-PAGE gels of haemolymph proteins, to identify soft bodied wood boring insect larvae such as the Asian longhorn beetle, Anoplophora glabripennis (Motscholsky, 1853) (Coleoptera: Cerambycidae). Due to stringent import restrictions and difficulty in obtaining licences to work with these organisms, we opted to work with four species of scarab beetle, Mecynorhina polyphemus (Fabricius, 1781), Pachnoda sinuata (Fabricius ,1775), Eucidella shiratica (Csiki, 1909) and Eucidella shultzeorum (Kolbe, 1906) which have near identical larval morphologies. RESULTS We show that this technology when combined with an advanced pattern matching system [Digital Automated Identification SYstem - DAISY] can classify soft bodied insect larvae, which are morphologically nearly identical, to species at a level of accuracy which is in excess of 98%. The study also indicates that the technology copes well with noisy data and small training sets. CONCLUSION The experience gained in undertaking this study gives us confidence that we will be able to develop a field deployable system in the medium term. We believe that as a high throughput identification tool, this technology is superior to competitor technologies (for example, finger printing of imaged DNA gels) in terms of speed, cost and ease of use; and therefore, it is suitable for low cost deployment in the field.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/ps.4766

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