3 years ago

Auto‐regulated Protein Assembly on a Supramolecular Scaffold

Peter B Crowley, Javier Pérez, Martin Rennie, Gavin Fox


Controlled protein assembly provides a means to regulate function. Supramolecular building blocks, including rigid macrocycles, have proven to be versatile triggers of protein assembly. Here, we show that sulfonato‐calix[8]arene (sclx8) mediates the formation of cytochrome c tetramers in solution. This tetramer spontaneously disassembles at ≥ 2 equivalents of sclx8 providing a remarkable example of "auto‐regulation". Using X‐ray crystallography we characterize in detail the sclx8 binding sites on cytochrome c. Crystal structures at different protein‐ligand ratios reveal varying degrees (up to ~35 %) of protein surface coverage by the calixarene and suggest a mechanism for oligomer disassembly. The solution structure of the oligomer was characterized by small angle X‐ray scattering. Overall, the data indicate calixarene‐controlled protein assembly and disassembly without the requirement for a competitive inhibitor, and point to protein encapsulation by a flexible macrocycle.

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201807490

DOI: 10.1002/anie.201807490

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