Stem Cell Research
Stem Cell Research
4 years ago

Generation of a human control PBMC derived iPS cell line TUSMi001-A from a healthy male donor of Han Chinese genetic background.

Jian Zhao, Ying Wang, Zhongmin Liu, Chaowen Yang, Gang Li, Ying Lei, Yue Zhang, Jing Zhang, Jing Lu
A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. In the studies of disease mechanism, the iPSC line can be used as a control.

Publisher URL: http://doi.org/10.1016/j.scr.2017.10.006

DOI: 10.1016/j.scr.2017.10.006

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