Stem Cell Research
Stem Cell Research
4 years ago

Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 *4/*17 variant which confers intermediate drug metabolizing activity.

Audrey Ncube, Martina Bohndorf, Lucas-Sebastian Spitzhorn, Wasco Wruck, James Adjaye, Jürgen Enczmann
SIX2-positive renal cells isolated from urine from a 51year old male of African origin bearing the CYP2D6 *4/*17 variant were reprogrammed by nucleofection of a combination of two episomal-based plasmids omitting pathway (TGFβ, MEK and GSK3β) inhibition. The induced pluripotent stem cells (iPSCs) were characterized by immunocytochemistry, embryoid body formation, DNA-fingerprinting and karyotype analysis. Comparative transcriptome analyses with human embryonic stem cell lines H1 and H9 revealed a Pearson correlation of 0.9243 and 0.9619 respectively.

Publisher URL: http://doi.org/10.1016/j.scr.2017.10.004

DOI: 10.1016/j.scr.2017.10.004

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