Stem Cell Research
Stem Cell Research
4 years ago

Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9.

Xinrong Ke, Yanli Liu, Fan Yang, Yin-Xiong Li, Liangqing Hong, Yingying Xu, Fang Yuan, Keyu Lai, Ge Gao, Yuhang Wu, Dongsheng Guo
The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

Publisher URL: http://doi.org/10.1016/j.scr.2017.08.016

DOI: 10.1016/j.scr.2017.08.016

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