Stem Cell Research
Stem Cell Research
4 years ago

Generation of iPSC line from patient with arrhythmogenic right ventricular cardiomyopathy carrying mutations in PKP2 gene.

Anna Malashicheva, Tatiana Pervunina, Natalia Yany, Aleksandr Khudiakov, Alexey Tomilin, Alexey Sergushichev, Anna Zlotina, Daria Kostina, Anna Kostareva
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed in vitro using immunofluorescence and RT-PCR analysis and in vivo by teratoma assay. The reported iPSC line could be useful tool for in vitro modeling of arrhythmogenic right ventricular cardiomyopathy.

Publisher URL: http://doi.org/10.1016/j.scr.2017.08.014

DOI: 10.1016/j.scr.2017.08.014

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