Stem Cell Research
Stem Cell Research
4 years ago

Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene.

Anna Malashicheva, Aleksandr Khudiakov, Alexey Tomilin, Alexey Sergushichev, Anna Zlotina, Tatiana Nikulina, Daria Kostina, Anna Kostareva, Alexandra Gudkova
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.

Publisher URL: http://doi.org/10.1016/j.scr.2017.08.015

DOI: 10.1016/j.scr.2017.08.015

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