Stem Cell Research
Stem Cell Research
4 years ago

Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background.

Jian Zhao, Ying Wang, Danyi Huang, Gang Li, Ying Lei, Yue Zhang, Jing Zhang
Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies. Resource table.

Publisher URL: http://doi.org/10.1016/j.scr.2017.08.008

DOI: 10.1016/j.scr.2017.08.008

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