Stem Cell Research
Stem Cell Research
4 years ago

Generation of two MEN1 knockout lines from a human embryonic stem cell line.

Feima Wu, Yanli Liu, Fan Yang, Aynisahan Ruzi, Yin-Xiong Li, Liangxue Lai, Fang Yuan, Keyu Lai, Haikun Liu, Han Wu, Yuanqi Zhuang, Yuhang Wu, Dongsheng Guo
The MEN1 gene is cytogenetically located at 11q13.1 and encodes the nuclear protein menin, which is involved in cell proliferation, apoptosis, differentiation, and metabolism. Here, we generated two MEN1 knockout human embryonic stem cell lines, WAe001-A-4 and WAe001-A-5, by targeting exon-2 and exon-9 of MEN1 using the CRISPR/Cas9 technique. These cell lines maintained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype. These human MEN1-mutated cell lines not only enlarge the pool of lab resources but also provide ideal models to dissect the detailed physio-pathological roles of the menin protein.

Publisher URL: http://doi.org/10.1016/j.scr.2017.06.005

DOI: 10.1016/j.scr.2017.06.005

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