Stem Cell Research
Stem Cell Research
5 years ago

Generation of three miR-122 knockout lines from a human embryonic stem cell line.

Jiawang Tao, Feima Wu, Yanli Liu, Fan Yang, Yan Chen, Ruzi Wei, Yin-Xiong Li, Kepin Wang, Liangxue Lai, Fang Yuan, Keyu Lai, Tingcai Pan, Nasir Abbas, Yuhang Wu, Yuanqi Zhuang, Dongsheng Guo
miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype.

Publisher URL: http://doi.org/10.1016/j.scr.2017.06.004

DOI: 10.1016/j.scr.2017.06.004

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