5 years ago

Generation of three miR-122 knockout lines from a human embryonic stem cell line.

Jiawang Tao, Feima Wu, Yanli Liu, Fan Yang, Yan Chen, Ruzi Wei, Yin-Xiong Li, Kepin Wang, Liangxue Lai, Fang Yuan, Keyu Lai, Tingcai Pan, Nasir Abbas, Yuhang Wu, Yuanqi Zhuang, Dongsheng Guo
miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype.

Publisher URL: http://doi.org/10.1016/j.scr.2017.06.004

DOI: 10.1016/j.scr.2017.06.004

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.