3 years ago

Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52bp deletion and the ASXL1 p.R693X mutation.

Jaroslaw Sochacki, Rodrigo Madeiro da Costa, Martín H Bonamino, Mariana D'Andrea, Mayra Carneiro, Sylvie Devalle, Stevens Rehen, Cristiana Solza, Marcelo Reis, Ilana R Zalcberg, Adelmo Daumas, Telma Padilha, Bárbara Monte-Mór, Cintia E Gomez Limia
Peripheral blood sample was donated by a 61years old female patient diagnosed with acute myeloid leukemia secondary to a primary myelofibrosis harboring the 52-bp deletion in the CALR gene (c.1092_1143del, p.L367fs*46) and the R693X mutation in the ASXL1 gene (c.2077C>T, p.R693X). CD34+ cells were isolated from the sample and subjected to the reprogramming procedure by using the Sendai virus carrying the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc. iPS colonies generated retained the original mutations and displayed all the features of bona fide iPS cells.

Publisher URL: http://doi.org/10.1016/j.scr.2017.08.006

DOI: 10.1016/j.scr.2017.08.006

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