4 years ago

ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.

Jinqi Fan, Ye Yang, Xingjian Xu, Yiming Bao, Wanzhu Zuo, Fan Wang, Dong Zou, Lin Xia, Jiabao Cao, Wenming Zhao, Zhennan Wang, Yang Zhang, Jingfa Xiao, Songnian Hu, Xiaojiao Han, Man Li, Jian Sang, Zhang Zhang, Lili Hao, Guangyi Niu
Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and non-model organisms.

Publisher URL: http://doi.org/10.1093/nar/gkx875

DOI: 10.1093/nar/gkx875

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