Prof Robert Sutherland is primarily responsible for the development of methodology behind the ISHAGE Guidelines for the enumeration of viable CD34+ cells by flow cytometry for use in the peripheral blood transplant setting. This technique is used worldwide in Clinical Flow Cytometry laboratories. Prof Sutherland has performed scores of talks at International Symposia and other events on this topic and an introduction for the American Association of Blood Bankers (AABB) is available to be viewed on YouTube.
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Prof D. Robert Sutherland
Prof D. Robert Sutherland is a professor at the University of Toronto. He has published over 100 peer-reviewed articles, 15 Reviews, and 15 Technical Monographs. He is an expert in CD34+ cell enumeration and detection of Paroxysmal Nocturnal Hemoglobinuria by flow cytometry. He became an Assistant Professor in 1989 (Dept of Medicine, University of Toronto), Associate Professor in 1997, and Full Professor in 2009. His early research interests included studying the structural characteristics of variety of cell surface molecules associated with normal and malignant cellular phenotypes including CD34. His work studying the CD34 antigen led to the development of a new Flow Cytometric method to enumerate CD34+ cells. In 1996 this method evolved into a clinical Guideline for ISHAGE (Int’l Soc Hematotherapy and Graft Evaluation). The ‘single platform’ variant of the ISHAGE Guidelines is the most widely used method Worldwide to assess graft adequacy in the Bone Marrow Transplant setting and is embodied in several National and International Guidelines for graft assessment by Flow Cytometry. This work led to the Wallace H. Coulter Distinguished Lecture Award in 2006, “to recognize lifetime contribution to the science, education and practice of Clinical Cytometry.” As Technical Director of the UHN Clinical Flow Cytometry Laboratory at Toronto General Hospital, he develops new Flow Cytometric assays for deployment in the clinical laboratory. His group developed assays for the detection of Glyco-phosphatidyl-inositol (GPI)-linked structures that are lacking in Paroxysmal Nocturnal Hemoglobinuria and related disorders like Aplastic Anemia. His group recently developed standardised highly sensitive flow assays to detect this disease on a variety of instrument types.
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