Plant, Cell and Environment
Plant, Cell and Environment
4 years ago

AtRAE1 is involved in degradation of ABA receptor RCAR1 and negatively regulates ABA signaling in Arabidopsis

Liang Zhang, Zhibin Liu, Xiangge Kong, Dekuan Li, Yi Yang, Jianmei Wang, Xiaoyi Li, Xufeng Li, Xiaoyu Wang, Ying Li
The phytohormone abscisic acid (ABA) plays an important role in regulating plant growth, development and adaption to various environmental stresses. Regulatory components of ABA receptors (RCARs, also known as PYR/PYLs) sense ABA and initiate ABA signaling through inhibiting the activities of protein phosphatase 2C (PP2C) in Arabidopsis. However, the way in which ABA receptors are regulated is not well known. A DWD protein AtRAE1 (for RNA export factor 1 in Arabidopsis), which may act as a substrate receptor of CUL4-DDB1 E3 ligase, is an interacting partner of RCAR1/PYL9. The physical interaction between RCAR1 and AtRAE1 is confirmed in vitro and in vivo. Overexpression of AtRAE1 in Arabidopsis causes reduced sensitivity of plants to ABA, while suppression of AtRAE1 causes increased sensitivity to ABA. Analysis of protein stability demonstrates that RCAR1 is ubiquitinated and degraded in plant cells and AtRAE1 regulates the degradation speed of RCAR1. Our findings indicate that AtRAE1 likely participates in ABA signaling through regulating the degradation of ABA receptor RCAR1.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/pce.13086

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