3 years ago

Hsp90α regulates ATM and NBN functions in sensing and repair of DNA double-strand breaks

Hsp90α regulates ATM and NBN functions in sensing and repair of DNA double-strand breaks
Paolo Ascenzi, Antonio Antoccia, Rosa Pennisi, Stefano Leone, Alessandra Masi
The molecular chaperone heat shock protein 90 (Hsp90α) regulates cell proteostasis and mitigates the harmful effects of endogenous and exogenous stressors on the proteome. Indeed, the inhibition of Hsp90α ATPase activity affects the cellular response to ionizing radiation (IR). Although the interplay between Hsp90α and several DNA damage response (DDR) proteins has been reported, its role in the DDR is still unclear. Here, we show that ataxia-telangiectasia-mutated kinase (ATM) and nibrin (NBN), but not 53BP1, RAD50, and MRE11, are Hsp90α clients as the Hsp90α inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) induces ATM and NBN polyubiquitination and proteosomal degradation in normal fibroblasts and lymphoblastoid cell lines. Hsp90α-ATM and Hsp90α-NBN complexes are present in unstressed and irradiated cells, allowing the maintenance of ATM and NBN stability that is required for the MRE11/RAD50/NBN complex-dependent ATM activation and the ATM-dependent phosphorylation of both NBN and Hsp90α in response to IR-induced DNA double-strand breaks (DSBs). Hsp90α forms a complex also with ph-Ser1981-ATM following IR. Upon phosphorylation, NBN dissociates from Hsp90α and translocates at the DSBs, while phThr5/7-Hsp90α is not recruited at the damaged sites. The inhibition of Hsp90α affects nuclear localization of MRE11 and RAD50, impairs DDR signaling (e.g., BRCA1 and CHK2 phosphorylation), and slows down DSBs repair. Hsp90α inhibition does not affect DNA-dependent protein kinase activity, which possibly phosphorylates Hsp90α and H2AX after IR. Notably, Hsp90α inhibition causes H2AX phosphorylation in proliferating cells, this possibly indicating replication stress events. Overall, present data shed light on the regulatory role of Hsp90α on the DDR, controlling ATM and NBN stability and influencing the DSBs signaling and repair. Model depicting the interplay between heat shock protein 90 (Hsp90α), ataxia-telangiectasia-mutated kinase (ATM), and nibrin (NBN). Hsp90α-ATM and Hsp90α-NBN form two complexes in unstressed cells. Upon IR-induced DNA double-strand breaks (DSBs), ATM becomes phosphorylated and active, and in turn it rapidly phosphorylates NBN and Hsp90α molecules present within the nucleus. The phSer343-NBN form dissociates from Hsp90α and relocalizes to the DSBs together with MRE11 and RAD50, where it promotes the DNA damage response.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/febs.14145

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