Live-cell imaging of cell signaling using genetically encoded fluorescent reporters

5 years ago

Live-cell imaging of cell signaling using genetically encoded fluorescent reporters

Jin Zhang, Sohum Mehta, Qiang Ni

Synergistic advances in fluorescent protein engineering and live-cell imaging techniques in recent years have fueled the concurrent development and application of genetically encoded fluorescent reporters that are tailored for tracking signaling dynamics in living systems over multiple length and time scales. These biosensors are uniquely suited for this challenging task, owing to their specificity, sensitivity, and versatility, as well as to the noninvasive and nondestructive nature of fluorescence and the power of genetic encoding. Over the past 10 years, a growing number of fluorescent reporters have been developed for tracking a wide range of biological signals in living cells and animals, including second messenger and metabolite dynamics, enzyme activation and activity, and cell cycle progression and neuronal activity. Many of these biosensors are gaining wide use and are proving to be indispensable for unraveling the complex biological functions of individual signaling molecules in their native environment, the living cell, shedding new light on the structural and molecular underpinnings of cell signaling. In this review, we highlight recent advances in protein engineering that are likely to help expand and improve the design and application of these valuable tools. We then turn our focus to specific examples of live-cell imaging using genetically encoded fluorescent reporters as an important platform for advancing our understanding of G protein-coupled receptor signaling and neuronal activity. Genetically encoded fluorescent reporters comprise an ever-expanding array of molecular tools that allow researchers to directly visualize different aspects of intracellular signaling within living cells. This review explores several recent innovations and advances in the design and application of genetically encoded fluorescent reporters to illuminate the mechanics of intracellular signaling pathways with increasing clarity.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/febs.14134