3 years ago

Mechanistic basis for increased human gene targeting by promoterless vectors—roles of homology arms and Rad54 paralogs

Mechanistic basis for increased human gene targeting by promoterless vectors—roles of homology arms and Rad54 paralogs
Shinta Saito, Aya Kurosawa, Noritaka Adachi
Gene targeting by homologous recombination provides the definitive tool for analyzing gene function. Promoterless vectors, which do not possess a promoter to drive marker gene expression, confer higher targeting efficiencies than conventional vectors due to the reduced number of drug-resistant clones. We here show that gene-targeting efficiency is typically ≥ 25% with the use of exon-trapping-type promoterless vectors in a human diploid cell line, Nalm-6. The efficiency of exon-trapping gene targeting was correlated with the level of target gene expression when a 2A peptide sequence was linked to the marker gene. Intriguingly, total arm length was not necessarily a determinant of targeting efficiency, as longer arms tend to enhance both homologous (targeted) and nonhomologous (nontargeted) integration of the vector; rather, the presence of an exon in the 5′ arm led to a decreased targeting efficiency. Strikingly, loss of Rad54 did not severely affect the targeting efficiency of exon-trap vectors. Moreover, additional deletion of the Rad54 paralog Rad54B had limited impact on the high-efficiency gene targeting. These results indicate that targeted integration occurs in human cells even when both Rad54 and Rad54B are missing. These studies provide additional important insight into the contribution of various DNA repair factors on the targeting mechanics. Promoterless vectors are advantageously used for gene targeting via homologous recombination. This paper investigates the utility and mechanistic basis of exon-trap-type promoterless vectors in human somatic cells. The findings not only provide possible future options for rational design of targeting vectors but also reveal an unexpectedly limited role of Rad54 and Rad54B in human cell gene targeting.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/febs.14137

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