Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
4 years ago

Single-Molecule Observation of The Photoregulated Conformational Dynamics of DNA Origami Nanoscissors

Yuu Kamada, Masayuki Endo, Yuki Suzuki, Hendrik Dietz, Kumi Hidaka, Elena M Willner, Tomoko Emura, Hiroshi Sugiyama
We demonstrate direct observation of the dynamic opening and closing behavior of photo-controllable DNA origami nanoscissors (NS) using high-speed AFM. First the conformational change between the open and closed state controlled by adjustment of salt concentration could be directly observed during AFM scanning. Then light-responsive moieties were incorporated into the NS to control the structural changes by irradiation. Using photo-switchable DNA strands, we created a photoresponsive NS variant and were able to distinguish between the open and closed conformations after respective irradiation with UV and visible (Vis) light via gel electrophoresis and AFM imaging. Additionally, these reversible changes in shape during photoirradiation were directly visualized using HS-AFM. Moreover, four photo-switchable NS were assembled into a scissor-actuator-like higher-order object whose configuration could be controlled by the open and close switching as induced by UV and Vis light irradiation.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708722

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