Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
4 years ago

A Strategy for Specific Fluorescence Imaging of Monoamine Oxidase A in Living Cells

Wen Shi, Xiaofeng Wu, Xiaohua Li, Huimin Ma
Monoamine oxidase (MAO) has two isoforms, MAO-A and MAO-B, which show different functions and thus selective fluorescence imaging of which is important for biological studies. Currently, however, specific detection of MAO-A remains a great challenge. Herein, we report a new strategy for specific imaging of MAO-A via designing fluorescent probes by combining the characteristic structure of the enzymatic inhibitor with propylamine as a recognition moiety. The high specificity of our representative probe is demonstrated by imaging MAO-A in different live cells such as SH-SY5Y (high level of MAO-A) and HepG2 (high level of MAO-B), as further validated by both control probe and western blot analyses. The superior specificity of the probe may benefit the accurate detection of MAO-A in complex biosystems. Importantly, in this work the use of the characteristic structure of an inhibitor may serve as a general strategy to design a specific recognition moiety of fluorescent probes for an enzyme.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708428

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