FEBS Journal
FEBS Journal
5 years ago

Ultra–high-resolution structure and charge-density analysis of high-potential iron–sulfur protein

Ultra–high-resolution structure and charge-density analysis of high-potential iron–sulfur protein
Kunio Miki, Kazuki Takeda
The high-potential iron–sulfur protein (HiPIP) is a small (~ 80 residues) soluble metalloprotein functioning as an electron carrier in photosynthetic bacteria. HiPIP has one Fe4S4 cluster at its molecular center. Its electronic structure is important for understanding electron transport. We recently succeeded in determining an ultra–high-resolution structure and analyzing the charge-density of HiPIP by using X-ray diffraction data at 0.48 Å resolution. The distribution of valence electrons in the iron–sulfur cluster and in the protein environment were clearly visualized, which is the first successful case for metalloproteins. In addition, a topological analysis of the charge density provided information about the electronic structure of the cluster. The charge-density analysis for the high-potential iron–sulfur protein (HiPIP) of a photosynthetic bacterium is successfully accomplished at an ultrahigh resolution of 0.48 Å. The valence electronic distributions and the atomic charges for the Fe4S4 cluster as well as the protein environment are experimentally determined as the first case for metalloproteins.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/febs.14036

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