3 years ago

Unprecedented Sensitivity in a Probe for Detection and Imaging of Cathepsin B: Chemiluminescence Microscopy Cell Images of Natively-Expressed Enzyme

Christoph Bauer, Doron Shabat, Michal Roth-Konforti
Until recently, chemiluminescence cell images could be obtained only using luciferase-activated probes. Moreover, chemiluminescence microscopy cell-imaging was never demonstrated for natively expressed enzymes like cathepsin B. Here we describe the design synthesis and evaluation of the first chemiluminescence probe for detection and imaging of cathepsin B. The probe activation mechanism relies on the release of a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using the probe, we obtained clear images of cancerous leukemia and colon cells. This is the first demonstration of chemiluminescence cell images obtained by a probe for a natively-expressed endogenous enzyme. We anticipate that the concept presented in this study will be broadly used to develop analogous probes for other important proteases relevant to biomolecular processes.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201709347

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