4 years ago

CODH-IV: A novel high efficiency CO-scavenging CO dehydrogenase with increased resistance to O2

Jae-Hun Jeoung, Lilith Domnik, Holger Dobbek, Sebastian Goetzl, Christophe Leger, Sebastien Dementin, Meriem Merrouch, Vincent Fourmond
CO dehydrogenases (CODHs) catalyse the reversible conversion between CO and CO2. Genomic analysis indicated that the metabolic functions of CODHs vary. The genome of Carboxydothermus hydrogenoformans encodes five CODHs (CODH-I - V), of which CODH-IV is found in a gene cluster near a peroxide reducing enzyme. Our kinetic and crystallographic experiments reveal that CODH-IV differs from other CODHs by characteristic properties: it has a very high affinity for CO, oxidizes CO at diffusion-limited rate over a wide range of temperatures, and is more tolerant to oxygen than CODH-II. Thus, our observations support the idea that CODH-IV is a CO scavenger in defence against oxidative stress and highlight that CODHs are more diverse in terms of reactivity than expected.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201709261

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