3 years ago

Engineered Fluorine Metabolism and Fluoropolymer Production in Living Cells

Engineered Fluorine Metabolism and Fluoropolymer Production in Living Cells
Michelle C. Y. Chang, Benjamin W. Thuronyi, Thomas M. Privalsky
Fluorine has become an important element for the design of synthetic molecules for use in medicine, agriculture, and materials. Despite the many advantages provided by fluorine for tuning key molecular properties, it is rarely found in natural metabolism. We seek to expand the molecular space available for discovery through the development of new biosynthetic strategies that cross synthetic with natural compounds. Towards this goal, we engineered a microbial host for organofluorine metabolism and show that we can achieve the production of the fluorinated diketide 2-fluoro-3-hydroxybutyrate at approximately 50 % yield. This fluorinated diketide can be used as a monomer in vivo to produce fluorinated poly(hydroxyalkanoate) (PHA) bioplastics with fluorine substitutions ranging from around 5–15 %. This system provides a platform to produce mm flux through the key fluoromalonyl coenzyme A (CoA) building block, thereby offering the potential to generate a broad range of fluorinated small-molecule targets in living cells. Live action: An engineered microbial host for organofluorine metabolism can produce a fluorinated diketide at around 50 % yield. The diketide can be used as a monomer to produce fluorinated poly(hydroxyalkanoate) bioplastics in vivo with fluorine substitution of up to 15 %. This system provides a platform to generate a broad range of fluorinated small-molecule targets in living cells.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201706696

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